Fig. 6. Activation of the Nrf2/HO-1 signaling pathway by I6CA in V79-4 cells. (A) The cells were pretreated with 300 μM I6CA for 1 h and then treated with or without 1 mM H₂O₂ for 24 h. Equal amounts of proteins were separated by SDS-polyacrylamide gel electrophoresis and subjected to Western blot analysis of the listed proteins. Actin was used as an internal control. (B) The cells cultured under the same conditions as in (A) were lysed, and the HO-1 activity was calculated using a commercial kit according to the manufacturer's procedure. The measurements were made in triplicate, and the values are expressed as the mean ± SD (**p<0.05 and ***p<0.001 compared with the control group).